Dipartimento di Ingegneria
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Current Position
PhD Student, University of Pavia, Italy
Academic Degree
| 2018 |
PhD in Bioengineering and Bioinformatics at the University of Pavia, Italy Thesis: Computational and experimental methods for metabolic engineering: applications in Escherichia coli and Bacillus subtilis. |
| 2014 |
Master Degree in Bioengineering at the University of Pavia, Italy. Thesis: Studio del silenziamento di geni target mediante sRNA in circuiti sintetici e pathway metabolici. |
| 2011 |
Bachelor Degree in Biomedical Engineering at the University of Cagliari, Italy. Thesis: Analisi modellistica del processo di crioconservazione di sospensioni cellulari. |
Honorary Appointment:
| Gen-May 2017 |
Visiting researcher, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark (DTU). |
Research Interests:
- Synthetic Biology in bacteria:
- Rational design and characterization of synthetic genetic circuits
- Mathematical modelling of biological systems
- Metabolic Engineering:
- Prediction and optimization of metabolic phenotype of microrganisms using genome-scale models
- In-vivo analysis of growth and metabolic behavior of E. coli and B. subtlis wild-type and mutant strains
Academic Activities:
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2017
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Co-supervisor of the thesis: "Strategie di ingegneria metabolica per ottimizzare la produzione di etanolo in Escherichia coli: studio in silico mediante modelli genome-scale e test in vivo tramite knockout genico" of Davide De Marchi, Biomedical Engineering Master's Degree. |
| 2015 | Co-supervisor of the thesis: "Valutazione di metodi computazionali per la predizione di flussi metabolici mediante modelli genome-scale" of Simone Maestri, Biomedical Engineering Master's Degree. |
Publications:
- Massaiu I, Pasotti L, Sonnenschein N, Rama E, Cavaletti M, Magni P, Calvio C and Herrgård M J. "Integration of enzymatic data in Bacillus subtilis genome-scale metabolic model improves phenotype predictions and enables in silico design of poly- γ-glutamic acid production strains". Microbial Cell Factories, 18:3 (2019).
- Casanova M, Pasotti L, Zucca S, Politi N, Massaiu I, Calvio C, Cusella De Angelis MG, Magni P. "A BioBrick(TM)-compatible vector for allelic replacement using the xylE gene as selection marker". Biological Procedures Online (2016).
- Massaiu I, Pasotti L, Casanova M, Politi N, Zucca S, Cusella De Angelis MG, Magni, P. (2015). Quantification of the gene silencing performances of rationally-designed synthetic small RNAs. Systems and synthetic biology, 9(3), 107-123 (2015).
- Pasotti L, Zucca S, Casanova M, Politi N, Massaiu I, Mazzini G, ... & Magni P. (2015, August). "Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology" IEE-EMBC (2015)